Identification of phosphorylation sites in βPIX and PAK1

Identification of phosphorylation sites in PIX and PAK1

phosphorylation sites in PIX and PAK1 Mark W. Mayhew1, Erin D. Jeffery2, Nicholas E. Sherman3, Kristina Nelson3, Joy M. Polefrone2, Stephen J. Pratt1, Jeffrey Shabanowitz2, J. Thomas Parsons4, Jay W. Fox2, Donald F. Hunt2,5 and Alan F. Horwitz1,* Departments of 1Cell Biology, 2Chemistry, 3W. M. Keck Biomedical Mass Spectrometry Laboratory, 4Microbiology, and 5Pathology, University of Virginia, ...

Identification of phosphorylation sites in GIT1.

G protein-coupled receptor kinaseinteracting protein 1 (GIT1) was originally identified as an ADP ribosylation factor GTPase-activating protein (ARF-GAP) that binds Gprotein-coupled receptor kinases (GRKs) and regulates membrane trafficking (Premont et al., 1998). Subsequent studies have shown a much broader function for GIT1 and GIT2/PKL as regulators of migrationrelated processes, including a...

Identification and functional analysis of SOX10 phosphorylation sites in melanoma

The transcription factor SOX10 plays an important role in vertebrate neural crest development, including the establishment and maintenance of the melanocyte lineage. SOX10 is also highly expressed in melanoma tumors, and SOX10 expression increases with tumor progression. The suppression of SOX10 in melanoma cells activates TGF-β signaling and can promote resistance to BRAF and MEK inhibitors. S...

Identification and Characterization of MAPK Phosphorylation Sites in Runx2

Molecular modeling of DICER and identification of phosphorylation sites

RNA interference (RNAi) is a post-transcriptional process initiated by double-stranded RNA molecules that degrade a complementary target RNA. In the first step, long double-stranded RNA molecules are chopped into shorter duplexes by an endonuclease dubbed DICER. This work focused on the identification of phosphorylation sites in DICER and proceeded towards comparative modeling. The domain of un...